PHENOL COEFFICIENT TEST OF COMBINATION EXTRACT OF LEAVES AND PEEL OF CITRUS HYSTRIX DC IN VITRO AS AN ALTERNATIVE ANTISEPTIC

Abstract

Citrus hystrix DC (C.hystrix) contains several antimicrobial compounds that have the potential as antiseptic candidates. This study reports the effectiveness of C.hystrix leaf and fruit peel (LPE) combination extract against several microbial isolates in vitro through a phenol coefficient test (KF). KF test on C.hystrix LPE suspension, 70% alcohol control, 5% phenol with test microbe, in 1:20 dilution; 1:30; 1:40; 1:50; 1:60; 1:70; 1:80; 1:90; 1:100; 1:110; 1:150; 1:200; 1:250 which was observed at 5, 10, and 15 minutes and the KF value was calculated. The effective KF value is 5%=1 phenol equivalent. The results showed that the KF value of the control and LPE C.hystrix treatments for Staphylococcus aureus = 1.25; Escherichia coli=1.00; Pseudomonas aeruginosa=1.00; and Candida albicans= 1.00. In conclusion, the combination of leaf extract and C.hystrix fruit peel has effectiveness as an antimicrobial which has the potential to be used as an alternative antiseptic.